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KMID : 1377020220190020363
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Tissue Engineering and Regenerative Medicine
2022 Volume.19 No. 2 p.363 ~ p.375
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Bonding of Flexible Membranes for Perfusable Vascularized Networks Patch
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Hong So-Young
Song Ye-Jin
Choi Jae-Soon
Hwang Chang-Mo
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Abstract
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BACKGROUND: In vitro generation of three-dimensional vessel network is crucial to investigate and possibly improve vascularization after implantation in vivo. This work has the purpose of engineering complex tissue regeneration of a vascular network including multiple cell-type, an extracellular matrix, and perfusability for clinical application.
METHODS: The two electrospun membranes bonded with the vascular network shape are cultured with endothelial cells and medium flow through the engineered vascular network. The flexible membranes are bonded by amine-epoxy reaction and examined the perfusability with fluorescent beads. Also, the perfusion culture for 7 days of the endothelial cells is compared with static culture on the engineered vascular network membrane.
RESULTS: The engineered membranes are showed perfusability through the vascular network, and the perfused network resulted in more cell proliferation and variation of the shear stress-related genes expression compared to the static culture. Also, for the generation of the complex vascularized network, pericytes are co-cultured with the engineered vascular network, which results in the Collagen I is expressed on the outer surface of the engineered structure.
CONCLUSION: This study is showing the perfusable in vitro engineered vascular network with electrospun membrane. In further, the 3D vascularized network module can be expected as a platform for drug screening and regenerative medicine.
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KEYWORD
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Membrane bonding, Vascular networks, Complex tissue generation, Perfusion culture
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